Sulfonylcalix[4]arenetetrasulfonate as pre-column chelating reagent for selective determination of aluminum(III), iron(III), and titanium(IV) by ion-pair reversed-phase high-performance liquid chromatography with spectrophotometric detection
Abstract
Sulfonylcalix[4]arenetetrasulfonate (SO2CAS) has been examined as a pre-column chelating reagent for ultratrace determination of metal ions by ion-pair reversed-phase high-performance liquid chromatography with spectrophotometric detection. Metal ions were converted into the SO2CAS chelates in an acetic buffer solution (pH 4.7). The chelates were injected onto a n-octadecylsilanized silica-type Chromolith™ Performance RP-18e column and were eluted using a methanol (50 wt.%)-water eluent (pH 5.6) containing tetra-n-butylammonium bromide (7.0 mmol kg−1), acetate buffer (5.0 mmol kg−1), and disodium ethylendiamine-N,N,N′,N′-tetraacetate (0.10 mmol kg−1). Under the conditions used, Al(III), Fe(III), and Ti(IV) were selectively detected among 21 kinds of metal ions [Al(III), Ba(II), Be(II), Ca(II), Cd(II), Co(II), Cr(III), Cu(II), Fe(III), Ga(III), Hf(IV), In(III), Mg(II), Mn(II), Mo(VI), Ni(II), Pb(II), Ti(IV), V(V), Zn(II), and Zr(IV)]. The detection limits on a 3σ blank basis were 8.8 nmol dm−3 (0.24 ng cm−3) for Al(III), 7.6 nmol dm−3 (0.42 ng cm−3) for Fe(III), and 17 nmol dm−3 (0.80 ng cm−3) for Ti(IV). The practical applicability of the proposed method was checked using river and tap water samples.
Sulfonylcalix[4]arenetetrasulfonate (SO2CAS) has been examined as a pre-column chelating reagent for ultratrace determination of metal ions by ion-pair reversed-phase high-performance liquid chromatography with spectrophotometric detection. Metal ions were converted into the SO2CAS chelates in an acetic buffer solution (pH 4.7). The chelates were injected onto a n-octadecylsilanized silica-type Chromolith™ Performance RP-18e column and were eluted using a methanol (50 wt.%)-water eluent (pH 5.6) containing tetra-n-butylammonium bromide (7.0 mmol kg−1), acetate buffer (5.0 mmol kg−1), and disodium ethylendiamine-N,N,N′,N′-tetraacetate (0.10 mmol kg−1). Under the conditions used, Al(III), Fe(III), and Ti(IV) were selectively detected among 21 kinds of metal ions [Al(III), Ba(II), Be(II), Ca(II), Cd(II), Co(II), Cr(III), Cu(II), Fe(III), Ga(III), Hf(IV), In(III), Mg(II), Mn(II), Mo(VI), Ni(II), Pb(II), Ti(IV), V(V), Zn(II), and Zr(IV)]. The detection limits on a 3σ blank basis were 8.8 nmol dm−3 (0.24 ng cm−3) for Al(III), 7.6 nmol dm−3 (0.42 ng cm−3) for Fe(III), and 17 nmol dm−3 (0.80 ng cm−3) for Ti(IV). The practical applicability of the proposed method was checked using river and tap water samples.
Article Outline
1. Introduction
2. Experimental section
2.1. Apparatus
2.2. Reagents and solutions
2.3. Procedure
3. Results and discussion
3.1. Pre-column chelation conditions
3.2. HPLC behavior of metal–SO2CAS chelates
3.3. Sensitivity, calibration data, and reproducibility
3.4. Interferences
3.5. Practical applications
4. Conclusion
Acknowledgements
References
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